Structure, Organization, and Formation of the Flock House Virus RNA Replication Complex

Structure, Organization, and Formation of the Flock House Virus RNA Replication Complex

4.11 - 1251 ratings - Source

Positive-strand RNA viruses are the largest genetic class of viruses and include many serious human and plant pathogens. RNA replication by eukaryotic positive-strand RNA viruses is universally associated with membranes and membrane rearrangements. However, crucial questions remain regarding the role of membranes in RNA replication, the structure and organization of the viral RNA replication complex, and the mechanisms of replication complex assembly. To study these questions we utilized the nodavirus flock house virus (FHV), which contains one of the smallest and simplest genomes of any animal virus. We found that the sole FHV replication factor, protein A, and RNA synthesis localize to the interiors of 50 nm membranous spherules between the inner and outer mitochondrial membranes, which thus are compartments for RNA synthesis. We performed three-dimensional electron microscope tomography imaging of the FHV spherules, which combined with biochemical results, suggest protein A forms a shell lining the spherule interior membrane. Additionally, we found that expression of protein A was not sufficient to induce spherule formation, but also required a replicatable RNA template and protein A with functional polymerase activity. These results appear relevant to many other positive-strand RNA viruses and support recently proposed structural and likely evolutionary parallels with retrovirus and double-stranded RNA viruses.The IMOD software suite was used for fiducial mark tracking and alignment. ... Volume segmentation was performed by manual tracing in the planes of highest resolution with the program Xvoxtrace [147]. The mitochondrial reconstructions were visualized using Analyze (Mayo Foundation, Rochester, MN), ImageJ ( Nationalanbsp;...

Title:Structure, Organization, and Formation of the Flock House Virus RNA Replication Complex
Author: Benjamin G. Kopek
Publisher:ProQuest - 2008

You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.

Once you have finished the sign-up process, you will be redirected to your download Book page.

How it works:
  • 1. Register a free 1 month Trial Account.
  • 2. Download as many books as you like (Personal use)
  • 3. Cancel the membership at any time if not satisfied.

Click button below to register and download Ebook
Privacy Policy | Contact | DMCA